Fig 1: Depletion of OTUD4 in IECs alters the expression of AMPs and reshapes gut microbiota.(A) Heat map of differentially expressed genes (DEGs) (P < 0.05 and |log2 Fold change|=1) in the transcriptomic profile of intestinal epithelial cells (IECs) from Otud4fl/fl (n = 2) and Vil-Cre;Otud4fl/fl (n = 2) mice that were given 2.5% DSS in drinking water for 2 d.(B) GO (gene ontology) analysis from the transcriptomic data obtained in (A).(C) A table of GO pathways associated with bacterial defense from the transcriptomic data obtained in (A).(D) GSEA analysis of antimicrobial peptides from the transcriptomic data obtained in (A). FDR, false discovery rate; ES, enrichment score.(E) Heatmap of the indicated genes related antimicrobial peptides from the transcriptomic data obtained in (A).(F) qRT-PCR analysis of the indicated genes of IECs from two groups of Otud4fl/fl (n = 6) and Vil-Cre;Otud4fl/fl (n = 6) mice that were given 2.5% DSS in drinking water for 2 d.(G) PCoA analysis from 16S rRNA sequencing in the feces of Otud4fl/fl (n = 4) and Vil-Cre;Otud4fl/fl (n = 5) mice that were given 2.5% DSS in drinking water for 0 d or 5 d.(H) Chao1 richness and Shannon diversity analysis of the 16S rRNA sequencing data obtained in (G).(I) Bacterial relative abundance from 16S rRNA sequencing data obtained in (G).*P ?< ?0.05, **P ?< ?0.01, ***P < 0.001 (Student's unpaired t-test in F, H). Graphs show mean ± S.D. (F, H). Data are representative of two (F) independent experiments.
Fig 2: Knockout of OTUD4 in IECs leads tohyper-resistancetoS.t. infection.(A) Survival of Otud4fl/fl (n = 7) and Vil-Cre;Otud4fl/fl (n = 9) mice that were injected with Salmonella typhimurium (S.t.) (2 × 107 c.f.u. per mouse) by gavage.(B) Body weight change of Otud4fl/fl (n = 12) and Vil-Cre;Otud4fl/fl (n = 12) mice treated as in (A).(C) A representative image and the lengths of colons of Otud4fl/fl (n = 12) and Vil-Cre;Otud4fl/fl (n = 12) mice treated as in (A) and sacrificed at day 6.(D) Representative images of HE-stained colon sections of Otud4fl/fl and Vil-Cre;Otud4fl/fl mice treated as in (A) and sacrificed at day 6.(E) Bacterial counts (CFU/g) of feces, cecum, liver, and spleen from Otud4fl/fl (n = 5) and Vil-Cre;Otud4fl/fl (n = 5) mice treated as in (A).(F) qRT-PCR analysis of expression levels of the indicated genes of IECs from Otud4fl/fl (n = 6) and Vil-Cre;Otud4fl/fl (n = 6) mice at day 2 after injection of S.t. (2 × 107 c.f.u. per mouse) by gavage.∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 (Log-rank test in A, Student's unpaired t-test in B, C, E, F). Graphs show mean ± S.D. (B, C, E, F). Red and black scale bars represent 2 cm and 400 μm, respectively (C, D). Data are representative of two independent experiments (A, D–F) or combined two independent experiments (B, C).
Fig 3: OTUD4 inhibits the expression of AMPs by targeting MyD88.(A) Whole-cell extract of IECs from wild-type C57BL/6 mice was immunoprecipitated with control IgG or anti-OTUD4 and subjected to immunoblot analysis.(B) Pulldown (with GST beads and GST-K63-Ub-TUBE) and immunoblot analysis of IECs from Otud4fl/fl (n = 2) and Vil-Cre;Otud4fl/fl (n = 2) mice that were uninduced, given 2.5% DSS in drinking water for 2 days or infected with S.t. (2 × 107 c.f.u. per mouse) by gavage for 2 days. TUBE, tandem ubiquitin binding entity.(C) Immunoblot analysis of the indicated proteins in IECs from Otud4fl/fl (n = 2) and Vil-Cre;Otud4fl/fl (n = 2) mice treated as in (B).(D) qRT-PCR analysis of the indicated genes of intestinal organoids from Otud4fl/fl and Vil-Cre;Otud4fl/fl mice that were unstimulated or stimulated with LPS (10 µg/mL) or PGN (10 µg/mL) in the presence or absence of ST2825 (a MyD88 inhibitor, 10 µM) for 4 h.(E) qRT-PCR analysis of the indicated genes of intestinal organoids from Otud4fl/fl and Vil-Cre;Otud4fl/fl mice that were uninfected and infected with S.t. (MOI, 1:200) in the presence or absence of ST2825 (10 µM) for 2 h*P ?< ?0.05, **P ?< ?0.01, ***P < 0.001 (Student's unpaired t-test in D, E). Graphs show mean ± S.D. (D, E). Data are representative of two independent experiments (A, C, D, E) or three independent experiments (B).
Fig 4: OTUD4 in Paneth cells supportsDSS-induced colitis andS.t. infection.(A) A scheme of DSS-induced colitis (upper scheme) and body weight change (lower graph) of Otud4fl/fl (n = 7) and Def-Cre;Otud4fl/fl (n = 7) mice with 2.5% DSS for 5 days followed by normal sterile water for 2 days.(B) A representative image and the lengths of colons of Otud4fl/fl (n = 7) and Def-Cre;Otud4fl/fl (n = 7) mice treated as in (A).(C) Representative images of HE-stained colon sections of Otud4fl/fl and Def-Cre;Otud4fl/fl mice treated as in (A).(D) The pathology scores of Otud4fl/fl (n = 7) and Def-Cre;Otud4fl/fl (n = 7) mice treated as in (A).(E) qRT-PCR analysis of expression levels of the indicated genes of IECs from Otud4fl/fl (n = 6) and Def-Cre;Otud4fl/fl (n = 6) mice that were given 2.5% DSS in drinking water for 2 d.(F) Survival of Otud4fl/fl (n = 12) and Def-Cre;Otud4fl/fl (n = 8) mice that were injected with S.t. (2 × 107 c.f.u. per mouse) by gavage.(G) Body weight change of Otud4fl/fl (n = 12) and Def-Cre;Otud4fl/fl (n = 12) mice treated as in (F) and sacrificed at day 6.(H) A representative image and the lengths of colons of Otud4fl/fl (n = 12) and Def-Cre;Otud4fl/fl (n = 12) mice treated as in (F) and sacrificed at day 6.(I) Representative images of HE-stained colon sections of Otud4fl/fl and Def-Cre;Otud4fl/fl mice treated as in (F) and sacrificed at day 6.(J) Bacterial counts (CFU/g) of feces, cecum, liver, and spleen from Otud4fl/fl (n = 6) and Def-Cre;Otud4fl/fl (n = 6) mice treated as in (F).(K) qRT-PCR analysis of Def5a or Reg3g of IECs from Otud4fl/fl (n = 6) and Def-Cre;Otud4fl/fl (n = 6) mice at day 2 after injection of S.t. (2 × 107 c.f.u. per mouse) by gavage.*P ?< ?0.05, **P ?< ?0.01, ***P < 0.001 (Student's unpaired t-test in A, B, D, E, G, H, J, K, Log-rank test in F). Graphs show mean ± S.D. (A, B, D, E, G, H, J, K). Red and black scale bars represent 2 cm and 400 µm (B, C, H, I). Data are combined two independent experiments (A, B, D, F–H) or representative of two independent experiments (C, E, I, J, K).
Fig 5: Otud4 deficiency in IECs leads to increased resistance toDSS-inducedcolitis.(A) OTUD4 mRNA expression levels in colonic mucosa tissues from ulcerative colitis (UC) patients and healthy individuals (data from GEO database GDS3119) (n = 26).(B) A scheme of DSS-induced colitis (upper scheme) and body weight change (lower graph) of Otud4fl/fl (n = 14) and Vil-Cre;Otud4fl/fl (n = 14) mice treated with 2.5% DSS for 5 days followed by normal sterile water for 2 days.(C) A representative image and the lengths of colons of Otud4fl/fl (n = 14) and Vil-Cre;Otud4fl/fl (n = 14) mice treated as in (B).(D) Representative images of HE-stained colon sections of Otud4fl/fl and Vil-Cre;Otud4fl/fl mice treated as in (B).(E) The pathology scores of Otud4fl/fl (n = 14) and Vil-Cre;Otud4fl/fl (n = 14) mice treated as in (B).*P ?< ?0.05, **P ?< ?0.01, ***P < 0.001 (Student's unpaired t-test in A–C, E). Graphs show mean ± S.D. (A–C, E). Red and black scale bars represent 2 cm and 400 µm, respectively (C, D). Data are combined three independent experiments (B, C, E) or representative of three independent experiments (D).
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